Interfacial analysis of siRNA complexes with poly-ethylenimine (PEI) or PAMAM dendrimers in gene delivery
نویسندگان
چکیده
منابع مشابه
PAMAM dendrimers for efficient siRNA delivery and potent gene silencing.
Genuine, nondegraded PAMAM dendrimers self-assemble with siRNA into nanoscale particles that are efficient for siRNA delivery and induce potent endogenous gene silencing.
متن کاملAcetylation of PAMAM dendrimers for cellular delivery of siRNA
BACKGROUND The advancement of gene silencing via RNA interference is limited by the lack of effective short interfering RNA (siRNA) delivery vectors. Rational design of polymeric carriers has been complicated by the fact that most chemical modifications affect multiple aspects of the delivery process. In this work, the extent of primary amine acetylation of generation 5 poly(amidoamine) (PAMAM)...
متن کاملPAMAM dendrimers for siRNA delivery: computational and experimental insights.
Short double-stranded RNAs, which are known as short interfering RNA (siRNA), can be used to specifically down-regulate the expression of the targeted gene in a process known as RNA interference (RNAi). However, the success of gene silencing applications based on the use of synthetic siRNA critically depends on efficient intracellular delivery. Polycationic branched macromolecules such as poly(...
متن کاملDendrimers for siRNA Delivery
Since the discovery of the "starburst polymer", later renamed as dendrimer, this class of polymers has gained considerable attention for numerous biomedical applications, due mainly to the unique characteristics of this macromolecule, including its monodispersity, uniformity, and the presence of numerous functionalizable terminal groups. In recent years, dendrimers have been studied extensively...
متن کاملTracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery.
Poly(ethylenimine) (PEI) is one of a number of polycations that has been used successfully to transfer genes into living cells. Although PEI shows promise in the field of gene therapy, to date no rigorous proof of mechanism has been published regarding the fate of PEI/DNA administered for transfection. Here we show, by using fluorescent labeling and confocal microscopy, the paths of PEI/DNA com...
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ژورنال
عنوان ژورنال: Colloids and Surfaces B: Biointerfaces
سال: 2017
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2017.07.001